Biofabrication Under Fluorocarbon: A Novel Freeform Fabrication Technique to Generate High Aspect Ratio Tissue-Engineered Constructs
Identifieur interne : 000059 ( OpenAccess/Analysis ); précédent : 000058; suivant : 000060Biofabrication Under Fluorocarbon: A Novel Freeform Fabrication Technique to Generate High Aspect Ratio Tissue-Engineered Constructs
Auteurs : Andreas Blaeser ; Daniela F. Duarte Campos ; Michael Weber ; Sabine Neuss ; Benjamin Theek ; Horst Fischer ; Willi Jahnen-DechentSource :
- BioResearch Open Access [ 2164-7844 ] ; 2013.
Abstract
Bioprinting is a recent development in tissue engineering, which applies rapid prototyping techniques to generate complex living tissues. Typically, cell-containing hydrogels are dispensed layer-by-layer according to a computer-generated three-dimensional model. The lack of mechanical stability of printed hydrogels hinders the fabrication of high aspect ratio constructs. Here we present submerged bioprinting, a novel technique for freeform fabrication of hydrogels in liquid fluorocarbon. The high buoyant density of fluorocarbons supports soft hydrogels by floating. Hydrogel constructs of up to 30-mm height were generated. Using 3% (w/v) agarose as the hydrogel and disposable syringe needles as nozzles, the printer produced features down to 570-μm diameter with a lateral dispensing accuracy of 89 μm. We printed thin-walled hydrogel cylinders measuring 4.8 mm in height, with an inner diameter of ∼2.9 mm and a minimal wall thickness of ∼650 μm. The technique was successfully applied in printing a model of an arterial bifurcation. We extruded under fluorocarbon, cellularized alginate tubes with 5-mm outer diameter and 3-cm length. Cells grew vigorously and formed clonal colonies within the 7-day culture period. Submerged bioprinting thus seems particularly suited to fabricate hollow structures with a high aspect ratio like vascular grafts for cardiovascular tissue engineering as well as branching or cantilever-like structures, obviating the need for a solid support beneath the overhanging protrusions.
Url:
DOI: 10.1089/biores.2013.0031
PubMed: 24083093
PubMed Central: 3776616
Affiliations:
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Duarte Campos</name><affiliation><nlm:aff id="aff2"></nlm:aff></affiliation></author><author><name sortKey="Weber, Michael" sort="Weber, Michael" uniqKey="Weber M" first="Michael" last="Weber">Michael Weber</name><affiliation><nlm:aff id="aff2"></nlm:aff></affiliation></author><author><name sortKey="Neuss, Sabine" sort="Neuss, Sabine" uniqKey="Neuss S" first="Sabine" last="Neuss">Sabine Neuss</name><affiliation><nlm:aff id="aff1"></nlm:aff></affiliation><affiliation><nlm:aff id="aff3"></nlm:aff></affiliation></author><author><name sortKey="Theek, Benjamin" sort="Theek, Benjamin" uniqKey="Theek B" first="Benjamin" last="Theek">Benjamin Theek</name><affiliation><nlm:aff id="aff4"></nlm:aff></affiliation></author><author><name sortKey="Fischer, Horst" sort="Fischer, Horst" uniqKey="Fischer H" first="Horst" last="Fischer">Horst Fischer</name><affiliation><nlm:aff id="aff2"></nlm:aff></affiliation></author><author><name sortKey="Jahnen Dechent, Willi" sort="Jahnen Dechent, Willi" uniqKey="Jahnen Dechent W" first="Willi" last="Jahnen-Dechent">Willi Jahnen-Dechent</name><affiliation><nlm:aff id="aff1"></nlm:aff></affiliation></author></analytic><series><title level="j">BioResearch Open Access</title><idno type="ISSN">2164-7844</idno><idno type="eISSN">2164-7860</idno><imprint><date when="2013">2013</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><title>Abstract</title><p>Bioprinting is a recent development in tissue engineering, which applies rapid prototyping techniques to generate complex living tissues. Typically, cell-containing hydrogels are dispensed layer-by-layer according to a computer-generated three-dimensional model. The lack of mechanical stability of printed hydrogels hinders the fabrication of high aspect ratio constructs. Here we present submerged bioprinting, a novel technique for freeform fabrication of hydrogels in liquid fluorocarbon. The high buoyant density of fluorocarbons supports soft hydrogels by floating. Hydrogel constructs of up to 30-mm height were generated. Using 3% (w/v) agarose as the hydrogel and disposable syringe needles as nozzles, the printer produced features down to 570-μm diameter with a lateral dispensing accuracy of 89 μm. We printed thin-walled hydrogel cylinders measuring 4.8 mm in height, with an inner diameter of ∼2.9 mm and a minimal wall thickness of ∼650 μm. The technique was successfully applied in printing a model of an arterial bifurcation. We extruded under fluorocarbon, cellularized alginate tubes with 5-mm outer diameter and 3-cm length. Cells grew vigorously and formed clonal colonies within the 7-day culture period. Submerged bioprinting thus seems particularly suited to fabricate hollow structures with a high aspect ratio like vascular grafts for cardiovascular tissue engineering as well as branching or cantilever-like structures, obviating the need for a solid support beneath the overhanging protrusions.</p></div></front><back><div1 type="bibliography"><listBibl><biblStruct><analytic><author><name sortKey="Vacanti, C" uniqKey="Vacanti C">C Vacanti</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Griffith, Lg" uniqKey="Griffith L">LG Griffith</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Choi, Nw" uniqKey="Choi N">NW Choi</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Rastegar, F" uniqKey="Rastegar F">F Rastegar</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Boucard, N" uniqKey="Boucard N">N 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Song</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Cohen, Dl" uniqKey="Cohen D">DL Cohen</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Khaing, Zz" uniqKey="Khaing Z">ZZ Khaing</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Matson, Jb" uniqKey="Matson J">JB Matson</name></author></analytic></biblStruct></listBibl></div1></back></TEI><affiliations><list></list><tree><noCountry><name sortKey="Blaeser, Andreas" sort="Blaeser, Andreas" uniqKey="Blaeser A" first="Andreas" last="Blaeser">Andreas Blaeser</name><name sortKey="Duarte Campos, Daniela F" sort="Duarte Campos, Daniela F" uniqKey="Duarte Campos D" first="Daniela F." last="Duarte Campos">Daniela F. Duarte Campos</name><name sortKey="Fischer, Horst" sort="Fischer, Horst" uniqKey="Fischer H" first="Horst" last="Fischer">Horst Fischer</name><name sortKey="Jahnen Dechent, Willi" sort="Jahnen Dechent, Willi" uniqKey="Jahnen Dechent W" first="Willi" last="Jahnen-Dechent">Willi Jahnen-Dechent</name><name sortKey="Neuss, Sabine" sort="Neuss, Sabine" uniqKey="Neuss S" first="Sabine" last="Neuss">Sabine Neuss</name><name sortKey="Theek, Benjamin" sort="Theek, Benjamin" uniqKey="Theek B" first="Benjamin" last="Theek">Benjamin Theek</name><name sortKey="Weber, Michael" sort="Weber, Michael" uniqKey="Weber M" first="Michael" last="Weber">Michael Weber</name></noCountry></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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